Turn the agitation on by pushing the Agitation
ON
soft key located to the right side of
the Quest Controller Unit display. If solvent is splashing up into the Upper Manifold,
readjust the height of the agitation. The Mix Speed Needle Valve located on the front
of the Quest Controller Unit may also be used to soften or slow the agitation by
turning the valve clockwise.
Agitate the resin in cleavage solution for
2
hours at room temperature.
Addition of Liquid
HF
Scavenger
(Methoxytrimethylsilane)
to
RVs
After the resin has agitated in the cleavage solution for
2
hours at room temperature,
Move the agitator up by pushing the Agitation UP soft key located to the right of the
Quest Controller Unit display.
Provide a low flow rate nitrogen sweep of the
RVs during the addition of cleavage
solution (HFPyridine in THF)
by
turning the Manifold Control Valves to "UTILITY
1"
and "METERED GAS". Gas will flow out of the Utility 1 port to the oil bubbler
Add
0.5
mL
of Me;SiOMe to all RVs. Remove the Luer Plug from the first RV Luer
Port (twist and pull) and insert the syringe needle below the Teflon lines of the male
Teflon RV fittings (below the stream of
N2) and dispense
0.5
mL
of Me3SiOMe
scavenger solution. Take care not to score the interior surfaces of the Luer Port with
the needle, as marring the surface can affect the sealing of the Luer Port. After
delivery, replace the Luer Plug (twist and push) and add scavenger to the remaining
9
RVs.
During the scavenge step, keep RVs under
a
nitrogen blanket by first turning the
Manifold Control Valves to "CLOSED" and "METERED GAS". Then turn both of
the Manifold Control Valves to "CLOSED".
Turn the agitator on by pushing the Agitation
ON
soft key located to the right side of
the Quest Controller Unit display. If solvent is splashing up into the Upper Manifold,
readjust the height of the agitation. The Mix Speed Needle Valve located on the front
of the Quest Controller Unit may also be used to soften or slow the agitation strokes
by turning the valve clockwise.
Agitate the resin in
cleavage/scavenger solution for
2
hours at room temperature.
C.
Product Collection
1.
Pre-weigh ten
20
mL
scintillation vials and place them in the Quest Scintillation Vial
Collection Rack.
2.
Remove the glass waste tank
and
dispose of the waste in accordance with all local
laws and regulations.
WARNING
-
Always wear gloves, eye protection, and proper clothing such as a lab
coat when working with the Quest.
3.
Rinse the bottom of the Lower Manifold Reactor Unit where the Teflon lines for
collection are located with THF and wipe clean.
4.
Place the Scintillation Vial Rack under the Reactor Unit. Raise the collection tray to
its upper most position by first pushing in the silver buttons on the Waste Tray
handles and sliding the Waste Tray up until the tops of the scintillation vials touch the
Argonaut Technologies
24 04/02/98
Part Number 102298
Quest Tutorial
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com
(33 pages)
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